Abstract: Ex-vivo Subnormothermic Oxygenated Machine Perfusion of Swine Forelimbs Enables Prolonged Graft Preservation Prior to Transplantation

RESULTS: Two recipients were euthanized within 2 weeks of DBMT due to neutropenic sepsis and posttransplant lymphoproliferative disorder but both VCAs remained viable up to experimental endpoint. M4515 (full MHC-mismatched recipient) has been off of all immunosuppression for 3 weeks without any evidence of rejection. M3815 (haplomatched) developed mixed chimerism transiently at 6 weeks after DBMT and corresponding MLR assays demonstrated decreased anti-donor responses; immunosuppression was then successfully withdrawn for a total of 5 weeks before rejection developed. Although the rejection episode could be reversed with steroid bolus and a tapering course of FK506, recurrence occurred after another 2 weeks off immunosuppression.